Haematic Potentials of Rhynchospore Corymbosa and Olax Subscorpioïdea Extract in Phenylhydrazine – induced Anaemic Rats

Authors

  • M’béfèhê Soro Agro-valuation Laboratory and Agroforestry, UFR Agroforestrie, Jean Lorougnon Guédé University (UJLoG), Ivory Coast
  • Joël Akakpo- Akue Laboratory of Pharmacodynamic and Biochemistry, UFR Biosciences, Université Félix Houphouët-Boigny (UFHB), Côte d’Ivoire
  • Jacques Auguste Alfred Bognan Ackah Agro-valuation Laboratory and Agroforestry, UFR Agroforestrie, Jean Lorougnon Guédé University (UJLoG), Ivory Coast
  • Jean-François Sibri Agro-valuation Laboratory and Agroforestry, UFR Agroforestrie, Jean Lorougnon Guédé University (UJLoG), Ivory Coast
  • Yayé Guillaume Yapi Agro-valuation Laboratory and Agroforestry, UFR Agroforestrie, Jean Lorougnon Guédé University (UJLoG), Ivory Coast
  • Tatiana Kangah Mireille Kple Laboratory of Pharmacodynamic and Biochemistry, UFR Biosciences, Université Félix Houphouët-Boigny (UFHB), Côte d’Ivoire
  • Sihi Ephrem Léangeovers Getheme Sanko Laboratory of biology, Agropastoral Management Institute, Péléforo Gbon Coulibaly university, Côte d’Ivoire

Keywords:

Antianemic activity, R. corymbose,, O. subscorpioïdea, sickle cell, decoction

Abstract

The decoction of the whole plant of Rhynchospora corymbosa and Olax subscorpioïdea leaves are used by the Local traditional healers in the department of Korhogo (Northern Côte d’Ivoire) to treat patients with sickle cell disease. This study was designed to assess, the antianemic potential of these plants extract. Anemia was induced with phenylhydrazine hydrochloride in rats. Animals were divided in normal (N), control (C), test (T), and reference (R) groups. T group included ill-induced Animals treated with R. corymbosa and O. subscorpioidea extracts, and R group ill-induced animals treated with vitamin B9 for anemic animals. Dosing was made as 1- day single dose repeated dose. In anemic animals, the production rate of RBC was significantly (p < 0.001) higher in T group as compared to C group. R. corymbosa whole plant and O. subscorpioidea leaves extracts have a haematic potential. The anti-anaemic effect may partly explain their use in patients with sickle cell disease who are affected by a deficiency of erythrocytes and its components.

References

Azokou A., Achi Y. et Koné W. (2016). Impact des tiques sur les

bovins métissés dans le nord de la Côte d’Ivoire. Bulletin of animal

heath production in africa, , 60(2) : 109-118

Bachir N. (2020). Contribution à l’étude des propriétés des huiles

essentielles extraites à partir des plantes médicinales utilisées contre

l’anémie. Mémoire de Master, Université de Biskra (Algérie), p 138.

Berger J. (2007). Hématotoxicité de la phénylhydrazine J. Appliqué.

Biomed , 5(2): 125-130.

Beucher G, Grossetti E, Simonet T, Leporrier M, Dreyfus M. (2011).

Anémie par carence martiale et grossesse. Prévention et traitement. J

Gynecol Obstet & Biol Reprod. 40(3):185-200.

Bla Kouakou et al.. (2015). J. Appl. Biosci. Étude

ethnopharmacologique des plantes antipaludiques utilisées chez les

Baoulé- N’Gban de Toumodi dans le Centre de la Côte d’Ivoire,

Journal of Applied Biosciences, 85:7775– 7783 ;

Bruneton J. (2009). Pharmacognosie-Phytochimie, plantes

médicinales, 4e éd., revue et augmentée, Paris, Tec & DocÉditions

médicales internationales, 1288 p: ISBN 978-27430-1188-8.

Cantrell CL, Berhow MA, Phillips BS, Duval SM, Weisleder D.

(2003). Vaughn SF Bioactive crude plant seed extracts from the

NCAUR oilseed repository. Phytomedicine, 10(4):325–333.

https://doi.org/10.1078/0944711 03322004820

Coulibaly A., Boua G.,N'guessan O. et Nahounou B. (2020).

Evaluation of Moringa Oleifera Lam Leaves (Moringaceae) Diets

against Induced Anemia in Wistar Rats, EAS journal of nutrition and

food science, 2 (3): 101- 106

De Benoist B, McLean E, Egli I, Gogswell M. (2008). Worldwide

prevalence of anemia 1993- 2005. WHO Global Database on

Anaemia. WHO.

Dupont C. (2017). Prévalence carence en fer. Elsevier, 24 : 45-48

Dupouy-Manescau N. (2020). La transfusion sanguine chez les

carnivores domestiques : étude rétrospective sur 162 chiens et 125

chats présents au CHUVAC ENVT entre 2013 et 2020. Thèse de

Docteur, l’Université Paul-Sabatier de Toulouse (France), 122 p.

Dzoyem JP, Tchuenguem RT, Kuiate JR, Teke GN, Kechia FA,

Kuete V. (2014). In Vitro and In Vivo antifungal activities of selected

Cameroonian dietary spices. BMC Complement Altern Med, ,

(1):58. https://doi.org/10.1186/1472- 6882-14-58

Ebrahimzadeh, M.A., Nabavi, S.M., Nabavi, S.F., Bahramian, F. &

Bekhradnia, A.R. Antioxidant and free radical scavenging activity of

H. Officinalis L. Var Angustifoluis, V. Odorata, B. hyrcana et C.

(2010). Speciosum. Pak. J. Pharm. Sci., 23 (1): 29-34.

Garandi B, Fankam AG, Nayim P, Wamba BEN, Mbaveng AT,

Kuete V. (2018). Anti-staphylococcal activity and antibioticmodulating

effect of Olax subscorpioidea, Piper guineense,

Scorodophloeus zenkeri, Fagara leprieurii, and Monodora myristica

against resistant phenotypes. Investig Med Chem Pharmacol, 1(2):1–

https://doi.org/10.31183/imcp.2018.00017

Gbenou D., Richard T., Pierre D., Michael F. et Mansourou M.

(2006). Etude des propriétés antianémiques de justicia secunda vahl

(acanthaceae) chez des rats de souche wistar. Pharm Méd Trad Afr,

(14) : 45-54.

Gnagne et al., (2017). Étude ethnobotanique des plantes médicinales

utilisées dans le traitement du diabète dans le Département de

Zouénoula (Côte d’Ivoire) Journal of Applied Biosciences , 113:

-11266

Gui, P. A., Bahi, C., Kamou, K. R., Tiekpa, W. J., Gnaléi, R. M.,

Djyh, N., & Coulibaly A. (2019). Study of antianemic properties of

Parquetina nigrescens (Apocynaceae) in wistar rats. The Journal of

Phytopharmacology, 8(5), 216-219.

Inkoto L, Bongo N, Kapepula M, Masengo A, Gbolo Z, Tshiama C,

Ngombe K, Iteku B, Mbemba F, Mpiana P. et Ngbolua K. (2018).

Microscopic features and chromatographic fingerprints of selected

congolese medicinal plants: Aframomum alboviolaceum (Ridley) K.

Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.)

Skeels, Emergent Life Sciences Research, 4: 1-10.

Itodo AU, Gafar MK, Hassan LG Dangoggo SM. (2011). Amino

acid estimation and phytochemical screening of Indigofera

astragolina leaves. J. Chem. Pharm. Res., 2011, 2(5):277-285. I

Kerharo J. & Bouquet.A., (1950). plantes médicinales et toxiques.de

la Côte d’Ivoire-Haut-Volta. Vigot – Frères, Paris (France),; p. 138

Konkon N., Simaga D., Adjoungova A., N’guessan.,Zirihi G. et

Kone B. (2006). Etude phytochimique de mitragyna inermis

(willd.) o. ktze (rubiaceae), plante à feuille antidiabetique, Pharm

Méd Trad Afr, 14 : 73-80 p.

Koua K., Irié N., Effo K., Kouakou S., Ayoman T., Tetchi A. et Yapi

H. (2018). Evaluation of Anti-inflammatory Activity of Crinum

scillifolium Extracts in Wistar Rats. International Journal of

Biochemistry and Biophysics , , 6(4): 77-82.

Lemonne, N., Lamarre, Y., Romana, M., Mukisi-Mukaza, M., HardyDessources,

M.-D., Tarer, V., et al. (2013). Does increased red blood

cell deformability raise the risk for osteonecrosis in sickle cell

anemia? Blood 121, 3054–6.

Leonard E. (1986). Is ethanol a neurotoxin ? The effects of ethanol on

neuronal structure and function. Alcoholism; 21 : 325-38.

Létapin C. (2016). Méthodologie de quantification des tannins

condensés dans les ressources tropicales. Rapport de stage, Université

des Antilles (Antilles), 27 p.

Levy T., De la cruz gongora V. et Villalpando S. (2016). Anemia:

Causes and Prevalence, Encyclopedia of Food and Health, 156-163

p.

Macheix, J.J., Fleuriet, A. & Jay-Allemand, C. (2005). Les composés

phénoliques des végétaux: un exemple de métabolites secondaires

d’importance écoomique. Presses Polytechniques et Universitaires

Romandes (PPUR) : Lausanne, Suisse.

Mpondo, M. E., Dibong, S.D., Ladoh, Y.C.F., Priso, R.J. & Ngoye,

A. (2012). Les plantes à phénols utilisées par les populations de la

ville de Douala. Journal of Animal and Plant Sciences, 15: 2083-

MSHP (2016), Enquête de prévalence parasitaire du paludisme et de

l’anémie, (EPPD- CI) p51

Odoma S, Zezi AU, Danjuma NM, Ahmed A, Magaji MG. (2016).

Elucidation of the possible mechanism of analgesic actions of butanol

leaf fraction of Olax subscorpioidea Oliv. J Ethnopharmacol,

:323–327. https://doi.org/10.1 016/j.jep.2016.12.052

Okoro OC. Aloke C. Ibiam UA. Obasi NA. Orji OU. Ogbonnia EC.

And al., (2021) Studies on Ethanol Extracts of Olax subscorpioidea

Against Carbon Tetrachloride-Induced Hepatotoxicity in Rats.,

Pakistan Journal of Biological Sciences: Volume 24 (6): 724-732,

Organisation des Nations unies pour l’alimentation et l’agriculture

(FAO). (2013). La situation mondiale de l’alimentation et de

l’agriculture en 2013 : mettre les systèmes alimentaires au service

d’une meilleure nutrition. Rome (Italie) : FAO. 114 p. [Visité le

/1/2018]. En ligne :

http://www.fao.org/docrep/018/i3300e/i3300e.pdf

Popoola TD, Awodele O, Omisanya A, Obi N, Umezinwa C, Fatokun

AA. (2016). Three indigenous plants used in anticancer remedies,

Garcinia kola Heckel (stem bark), Uvaria chamae P. Beauv. (root)

and Olax subscorpioidea Oliv. (root) show analgesic and anti-

inflammatory activities in animal models. J Ethnopharmacol,

:440–449. https://doi.org/10.1016/j.jep.2016. 09.046

Righetti A., Koua A. et Adiossan L. (2012). Etiology of anemia

among infants, school-aged children, and young non-pregnant

women in different settings of South-Central Cote d’Ivoire, Trop Med

Hyg, 87: 425–34

R-Tiendrebeogo et al., . (2019). Plantes sahéliennes adaptées dans la

récupération des terres dégradées et leurs usages pour la santé : cas de

la province du Soum au Nord du Burkina Faso . Journal of Animal &

Plant Sciences (J.Anim.Plant Sci. ISSN 2071-7024), Vol.41 (1):

-6783. https://doi.org/10.35759/JAnmPlSci.v41-1.4

Sheth A., Pawar A.,Mote C. et More C. . (2021). Antianemic activity

of polyherbal, Raktavardhak kadha,against phenylhydrazine- induced

anemia in rats. Journal of Ayurveda and Integrative Medicine, 12:

- 345 p.

Soro M, Akakpo-Akue J, Ackah JAAB, Sibri JF, Yapi YG, Kple

TKM. . (2021). Effect of Rhynchospora corymbosa and Olax

subscorpioïdea two plants used in the management of Korhogo sickle

cell disease. J Phytopharmacol 10(1):1-6

Stuart, M.J., and Nagel, R.L. (2004). Sickle-cell disease. Lancet 364:

–1360. Sugihara, K., Sugihara, T., Mohandas, N., and Hebbel,

R.P. Thrombospondin mediates adherence of CD36+ sickle

reticulocytes to endothelial cells. Blood, 80: 2634–42.

Turaskar A., More S., Sheikh R., Gadhpayle J., Bhongade S. L and

Shende V. (2013). Inhibitory potential of picrorhiza kurroa royle ex.

Benth extracts on phenylhydrazine induced reticulocytosis in rats.,

Academic Sciences, Asian Journal of Pharmaceutical and Clinical

Research, 2013, Vol 6, Suppl 2,

Vert C. (2019). prise en charge de l’anemie par carence martiale chez

des patients beneficiant d’une chirurgie colorectale carcinologique,

De la faculté des sciences pharmaceutiques de l’université paul

sabatier. Université talouse

Yamunadevi, M., Wesely, E.G. & Johnson, M. (2011).

Phytochemical studies on the terpenoids of medicinally important

plant Aerva lanata L. using HPTLC. A.Pacific J. of

Trop.Biomedicine. S220-S225.

Yenon A., Yapi H., Gnahoue G., Yapo A., Nguessan J. et Djaman A.

(2015). Anti- anaemic activity of aqueous and ethanolic extracts of

Entandrophragma angolense bark on phenylhydrazine- induced

anaemic rats. Asian Journal of Biochemical and Pharmaceutical

Research, 5(3): 12-21.

Zangeneh, M.M., Zangeneh, A., Salmani, S. et al., (2019). Protection

of phenylhydrazine-induced hematotoxicity by aqueous extract

of Ocimum basilicum in Wistar male rats. Comp Clin Pathol 28, 331–

https://doi.org/10.1007/s00580-018-2845-8

Downloads

Published

2023-01-17

How to Cite

Soro, M., Akue, J. A.-., Ackah, J. A. A. B., Sibri, J.-F., Yapi, Y. G., Mireille Kple, T. K., & Getheme Sanko, S. E. L. (2023). Haematic Potentials of Rhynchospore Corymbosa and Olax Subscorpioïdea Extract in Phenylhydrazine – induced Anaemic Rats . ESI Preprints, 1, 200. Retrieved from https://esipreprints.org/index.php/esipreprints/article/view/259

Issue

Section

Preprints